Journal: Frontiers in Pharmacology

Article Title: Blocking TRAIL-DR5 signaling pathway with soluble death receptor 5 fusion protein mitigates radiation-induced injury

doi: 10.3389/fphar.2023.1171293

Figure Lengend Snippet: sDR5-Fc blocked apoptosis in human small intestinal mucosal epithelial cells after irradiation. (A) Viability of human small intestinal mucosal epithelial cells at different radiation and administration doses. (B) Apoptosis percentage in human small intestinal mucosal epithelial cells at 24, 48 and 72 h after irradiation with or without 50 μg/mL sDR5-Fc. Quantitative data are shown as means ± SEM. * p ≤ 0.05, ** p ≤ 0.01, and *** p ≤ 0.001, as determined using one-way ANOVA and two-way ANOVA.

Article Snippet: Human small intestinal mucosal epithelial cells (CP-H039, Procell, China) were cultured in a complete medium for human small intestinal mucosal epithelial cells (CM-H039, Procell, China) and Intestinal Epithelioid Cell line No. 6 (IEC-6 cells) were cultured in Dulbecco’s Modified Eagle Medium (812619, gibco, United States) with 10% fetal bovine serum (FBS) (SV30208.02, cytiva HyClone, United States)and 1% Penicillin Streptomycin (15140-122, gibco, United States) and at 37°C under 5% CO 2 at atmosphere.

Techniques: Irradiation

Journal: International Journal of Molecular and Cellular Medicine

Article Title: The Expression Pattern and Clinicopathological Importance of Hsa_circ_000425 in Colorectal Cancer

doi: 10.22088/IJMCM.BUMS.9.4.266

Figure Lengend Snippet: Expression levels of hsa-circ_000425 in different colorectal cancer cell lines. The expression levels of hsa-circ_000425 in HCT8, SW480, SW620, and Lovo as cancerous cell lines, and normal colon epithelial cell line (NCM460) were evaluated .A significant down regulation of hsa-circ_000425 was observed in Sw620 (P < 0.01), Sw480 (P < 0.01), and Lovo (P < 0.001) cell lines. All values are given as mean ± SD of three independent experiments

Article Snippet: Cell culture Four CRC cell lines (SW480, SW620, HCT8, and Lovo) and NCM460 as normal human colon epithelial cell were purchased from the Cell Bank of Pasteur Institute (Tehran, IRAN).

Techniques: Expressing

Journal: Oncotarget

Article Title: Hypermethylation of NDN promotes cell proliferation by activating the Wnt signaling pathway in colorectal cancer

doi: 10.18632/oncotarget.17580

Figure Lengend Snippet: (A) Immunostaining of NDN protein in CRC tissue samples and normal colorectal tissues. (B) Expression analyses of NDN protein in 12 surgical CRC tissues and the paired normal intestine epithelial samples using Western blot. β-tubulin was used as a loading control. (C) qRT-PCR analysis of NDN expression in 21 paired colorectal cancer tissues; NDN was quantified relative to the matched adjacent no tumor tissues. Error bars represent means ± SD calculated from three parallel experiments. (D) Expression analyses of NDN protein and mRNA in the normal intestinal epithelial cell FHC and five CRC cell lines through Western blot and qRT-PCR. Each bar represents the mean ± SD of three parallel replicates. (E) Influence of NDN expression on overall survival through Kaplan-Meier analysis in 84 CRC patients.

Article Snippet: Human normal intestinal epithelial cell FHC and five CRC cell lines SW480, HT29, HCT116, LS174T, and RKO were obtained from the The Global Bioresource Center (ATCC, USA).

Techniques: Immunostaining, Expressing, Western Blot, Control, Quantitative RT-PCR